Methodology and resources

  • Transgenic mouse models, conditional knock-out, segment-specific invalidation
  • Immortalized cell lines and primary cell culture systems developed from micro-dissected nephron segments
  • Manipulation of gene expression in cell lines and primary cultures
  • Promoter analysis (in silico, in vitro)
  • In situ hybridization, advanced quantitative RT-PCR
  • Immunoblotting, immunoprecipitation, and immunohisto-/cyto-chemistry
  • Intracellular distribution studies: subcellular fractionation, immunogold, biotinylation
  • Ontogeny of transporters and regulators
  • Transport studies in cells and native tissues (Ussing chamber)
  • Mouse phenotyping: metabolic cages, special diets, pharmacology interventions
  • Multisystemic phenotyping: cardiovascular, osmoregulation & thirst
  • Comprehensive biochemical profiling on dedicated platform optimalized for rodent samples
  • Development and automation of ELISA
  • Water and solute transport in mouse model of peritoneal dialysis
  • Biobanking (all human): end-stage kidney samples (200+); kidney biopsies (500+); urine samples from isolated populations (N=6000); peritoneal biopsies (100+)
  • DNA cohorts: ADPKD (N=300); rare inherited kidney disorders (N=200); renal transplant (N=300); peritoneal dialysis (N=800)
  • High throughput analysis of human plasma and urine samples - GWAS
  • Shared resources through EU-funded EUNEFRON, Genecure, EUROSPAN and EURenOmics consortia