Methodology and resources
- Transgenic mouse models, conditional knock-out, segment-specific invalidation
- Immortalized cell lines and primary cell culture systems developed from micro-dissected nephron segments
- Manipulation of gene expression in cell lines and primary cultures
- Promoter analysis (in silico, in vitro)
- In situ hybridization, advanced quantitative RT-PCR
- Immunoblotting, immunoprecipitation, and immunohisto-/cyto-chemistry
- Intracellular distribution studies: subcellular fractionation, immunogold, biotinylation
- Ontogeny of transporters and regulators
- Transport studies in cells and native tissues (Ussing chamber)
- Mouse phenotyping: metabolic cages, special diets, pharmacology interventions
- Multisystemic phenotyping: cardiovascular, osmoregulation & thirst
- Comprehensive biochemical profiling on dedicated platform optimalized for rodent samples
- Development and automation of ELISA
- Water and solute transport in mouse model of peritoneal dialysis
- Biobanking (all human): end-stage kidney samples (200+); kidney biopsies (500+); urine samples from isolated populations (N=6000); peritoneal biopsies (100+)
- DNA cohorts: ADPKD (N=300); rare inherited kidney disorders (N=200); renal transplant (N=300); peritoneal dialysis (N=800)
- High throughput analysis of human plasma and urine samples - GWAS
- Shared resources through EU-funded EUNEFRON, Genecure, EUROSPAN and EURenOmics consortia